EN 49-1 pdf free download
EN 49-1 pdf free download. Wood preservatives – Determination of the protective effectiveness against Anobium punctatum (De Geer) by egg-laying and larval survival – Part 1: Application by surface treatment (Laboratory method).
2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN ISO 835, Laboratory glassware — Graduated pipettes (ISO 835) EN ISO 3696, Water for analytical laboratory use — Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled [SOURCE: EN 1001-2:2005, 4.71] 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) Note 1 to entry: Adapted from EN 1001–2:2005, 4.83. 4 Principle Depending on the test being carried out either: — on a set of test specimens of a susceptible wood species that is surface treated with a solution of the preservative, or — if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that are surface treated with a series of solutions in which the concentration of preservative is ranged in a given progression. The treated test specimens are exposed to gravid females of Anobium punctatum. The number of eggs laid, the number of eggs hatched and the numbers of surviving larvae are observed and compared with those in untreated control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test specimens. 5 Test materials 5.1 Biological material Anobium punctatum (De Geer) Adult males and females in good condition. Adults to be used in the test shall be collected at daily intervals from naturally infested wood or laboratory culture (see Annex C). Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.6); and then checked to ensure that they are undamaged, active and free from any infestation by mites. Determine the sex (see Annex B) of the collected and checked adults and place the males and females in separate containers. NOTE The proportion of males and females varies during the emergence period. 5.2 Products and reagents 5.2.1 Paraffin wax, for sealing the relevant faces of test specimens to be treated with solutions in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable. 5.2.2 Gelatin, for sealing the relevant faces of test specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and insoluble in the product under test. NOTE Sodium carboxy methyl cellulose, food grade, has been found to be suitable. 5.2.4 Water, complying with grade 3 of EN ISO 3696. 5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION — Do not use benzene or other solvents which pose a health risk. 5.2.6 Filter paper, ordinary quality, medium-fast grade. 5.2.7 Fine cloth of a suitable material with a mesh aperture of 0,3 mm to 0,6 mm for the preparation of the egg-laying zones. NOTE Cotton, linen and polyamide-gauze have been proven as suitable.EN 49-1 pdf download.
